Browse Catalog. Life Science Research Back. Life Science Research Explore all. Bio-Rad Products Explore all. Support Explore all. Clinical Diagnostics Explore all. Process Separations Explore all. Food Science Explore all. Bio-Rad Products Back. Life Science Education Explore all. About the Program Explore all. Corporate Explore all. About Bio-Rad Back. About Bio-Rad Explore all. Investor Relations Explore all. Incubate at room temperature for at least 5 minutes.
Absorbance will increase over time; samples should incubate at room temperature for no more than 1 hour. Measure absorbance at nm. Prepare three to five dilutions of a protein standard which is representative of the protein solution to be tested. The linear range of the assay for BSA is 1.
The linear range of the Standard and Microassay procedures when used in the microtiter plate format is slightly changed, since the ratio of sample to dye is modified. Filter through a Whatman 1 filter or equivalent to remove particulates. This diluted reagent may be used for about 2 weeks when kept at room temperature. The linear range of this microtiter plate assay is 0.
Mix the sample and reagent thoroughly using a microplate mixer. Alternatively, use a multi-channel pipet to dispense the reagent. Depress the plunger repeatedly to mix the sample and reagent in the well. Replace with clean tips and add reagent to the next set of wells. The linear range of the assay is 8. Welcome to ManualMachine. We have sent a verification link to to complete your registration. Log In Sign Up. Forgot password? Enter your email address and check your inbox. Please check your email for further instructions.
Enter a new password. Protein Assay. Section 1 Introduction The Bio-Rad Protein Assay, based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubilized protein.
The Bio-Rad Protein Assay is for research use only. You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts.
Inspect mode — when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents. Traditional semi-dry transfer system transfers proteins ranging from 10— kD in 15—60 minutes. Trans-Blot Turbo system delivers efficient and reproducible transfer of proteins up to kD in as little as 3 minutes.
The Trans-Blot Turbo system is a rapid protein transfer apparatus that can transfer protein to membrane in as little as 3 minutes. Trans-Blot Turbo transfer packs provide greater transfer efficiency in less time. The Trans-Blot SD semi-dry transfer cell allows fast, efficient, and economical blotting without a buffer tank or gel cassettes. You can create and edit multiple shopping carts Edit mode — allows you to edit or modify an existing requisition prior to submitting.
You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts Inspect mode — when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. My Account. Browse Catalog.
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