To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes xylene cyanol, bromophenol blue, and orange G to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far see figure GelPilot Loading Dye. QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters.
QIAvac Manifold with luer connectors. QIAvac 24 plus. DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
It is difficult to predict whether a DNA fragment larger than 10 kb can be efficiently recovered, because this depends on base composition as well as fragment size. However, please note that it will become less likely to recover your sample the larger the fragment size is. As we cannot guarantee recovery of fragments larger than the maximum cutoff size, we do not recommend to purify such fragments using QIAquick Kits.
Buffer PE did not contain ethanol. Ethanol must be added to Buffer PE concentrate before use. Repeat procedure with correctly prepared Buffer PE. DNA will only be eluted efficiently in the presence of low-salt buffer e. Elution efficiency is strongly dependent on the salt concentration and pH of the elution buffer. Contrary to adsorption, elution is most efficient under basic conditions and low salt concentrations. The maximum elution efficiency is achieved between pH 7.
When using water to elute, make sure that the pH is within this range. Elution buffer incorrectly dispensed. Add elution buffer to the center of the QIAquick membrane to ensure that the buffer completely covers the membrane. Please see a user-developed procedure below, which was kindly provided by J.
The rRNA bands were excised and treated as described above left lane or using 10 volumes of Buffer QG in step 3 right lane. The extracted RNA was then analyzed on a new formaldehyde agarose gel. Data kindly provided by J. By comparison, it should be possible to purify fragments longer than nucleotides using the QIAquick Gel Extraction Kit.
Note that recovery of single strand DNA is influenced to some degree also by factors such as base composition and secondary structure. It has to be determined empirically by the researcher if cleanup of single-stranded DNA with QIAquick columns yields satisfactory results. Under certain conditions, chaotropic agents present in all silica-based DNA purification methods can denature DNA fragments. This is a rare event that may be influenced by sequence characteristics such as the presence of inverted repeats or A—T-rich stretches.
Because salt and buffering agents promote renaturation of DNA strands, the following tips are recommended:. We always provide extra buffers in our kits so you can scale up reactions, add extra washes or allow for spillage. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste.
Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach. To ensure optimal diffusion, cut the gel slices as small as possible, and use 2 volumes of diffusion buffer per 1 volume of gel. Increasing incubation time protocol step 3 may result in higher yields.
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Looking for a quick way to design experiments? Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs. Log Out. Show More. Log in to see your account pricing. Kit Column. QIAquick Spin Columns. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples see figure " High recoveries from gels ". Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries.
Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges. To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes xylene cyanol, bromophenol blue, and orange G to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far see figure "GelPilot Loading Dye".
QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
Print Share. Please log in Show cart. Add to cart. This product is not intended for the diagnosis, prevention, or treatment of a disease. QIAquick and MinElute procedure. High recoveries from gels.
GelPilot Loading Dye. Spin column handling options — D. Spin column handling options — E. Spin column handling options — C. Spin column handling options — B. Spin column handling options — A. Reliable sequencing after gel extraction. The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold. Samples were analyzed on a 1. Samples were analyzed on a 3. M : pTZ- Hin fI markers. An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared.
QIAvac 24 plus. Manifold with luer connectors.
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